Dear Flowers, I would like to ask for informations about FACS-staining of CCR6 (chemokinereceptor 6). In particular, I am looking for a CCR6 positive celline for titration of CCR6 specfic antibodies and for possible pitfalls. Is the temperature and incubationtime of the CCR6 antibody (monoclonal) important? In the literature following staining procedure was described : Incubation with the first antibody for 1h at room temperature. In our lab we incubate the cells 15min on ice. Thank you very much Christa Institute for Pathology University of Würzburg Germany Christa Amrehn Institut fuer Pathologie Uni Wuerzburg Josef-Schneider Str. 2 97080 Wuerzburg 49/0931/201-3790 path132@mail.uni-wuerzburg.de
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:18 EST