Tony, A question . . . do you see no flow at all? If this is true, then check the lines going through the pinch plates on the back of the control valve. I used to find that after periods of disuse these tubes would be stuck together (crimped?), even after there was no pressure from the plate (hope this is clear). Alternatively, check that there is no blockage to the waste tank, and the waste tank is vented. If you're getting flow to the waste tank, but cannot get sample to flow, check the sample tube seal. Finally, you can also apply back pressure to the sipper . . . remove the drip-back vacuum sheath, place a bit of small tubing on the tip of a syringe, and attach that to the end of the sipper. Be careful not to torque the sipper - - this goes directly into the flow cell. Disrupting its alignment into the flow cell will dramatically effect your machine's alignment. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> Tony Schountz <tschount@mesastate.edu> 01/17/01 09:32PM >>> Our BD FACScan seems to have flow problems. It hasn't been used for a couple of months, so I think it's just clogged lines. But since we don't have a service contract I was hoping the group might have some suggestions as to get the lines unclogged. In the event this isn't the problem, what else should I check out? Thanks, Tony -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu
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