Dear Tessa Margaret Goodell's lab has an excellent website, with protocols at: http://www.bcm.tmc.edu/genetherapy/goodell/new_site/index2.html I believe Margaret and co-workers were the first to indentify side population cells. We have been looking at SP cells for over a year now and after a few early inconsitencies now seem to have a reliable technique. We have found that it is important to follow the Goodell protocol to the letter. Hope this helps Ian Titley PhD Leukaemia Research Fund Centre at the Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB UK Tel Direct: +44 (0)20 7970 6048 or Tel: +44 (0)20 7352 8133 ext5134 Fax: +44 (0)20 7352 3299 E-mail: iant@icr.ac.uk On Wed, 17 Jan 2001 18:04:39 +0100 Tessa Kerre <tessa.kerre@rug.ac.be> wrote: > > Hi all, > > > Some of my colleagues in the lab are trying to set up a protocol for > studying SP (side population) cells on a FACSVantage, using Hoechst > labelling. > They have been trying for a few weeks now (using mouse bone marrow > cells, but ultimately they want to use human cells), but have problems > with setting the UV right, and getting the typical SP pattern. Of course > they do not know whether the problem lies with their labelling or with > the cytometer settings. > > Are there people who have experience with this labelling, and can help > my colleagues with references and practical guidance. > Are there labs close to Belgium where they can come and learn at the > spot how to set the Vantage? > > Thanks a lot! > > Tessa Kerre > Ghent University Hospital - Immunology Department > Belgium >
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