in an old paper (Cytometry 8: 114, 1987) we described a method of cell-by-cell subtraction of autofluorescence using a straightforward fluorescence channel compensation procedure. For flow analyses this is useful and easy. In principle, you could do the same in a confocal microscope by a point-by-point subtraction of an image taken in the red, using green reagents. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Fri, 12 Jan 2001, Joseph Mathew Antony wrote: > > Hi > > I have been having a problem with autofluorescence of fish tissues and > cells. Does anybody know how to quench this? Suggestions will be warmly > appreciated.....Cheers > Joseph > Joseph Mathew Antony > Laboratory of Animal Health Biotechnology > Institute of Molecular Agrobiology > National University of Singapore > 1 Research Link, Singapore 117604 > > Tel: 8727472/8747474 > Fax: 8727518/8727007 > e mail: joseph@ima.org.sg > josephmatthew@hotmail.com > scip7297@nus.edu.sg > > " A journey of thousand miles begins with a single step " Confucious > > "You see things; and you say 'Why?' But I dream things that > never were; and I say 'Why not?'" -- George Bernard Shaw > > "Science is a great game. It is inspiring and refreshing.The playing field > is the universe itself" Isidor Isaac Rabi (US Physicist, Nobel Laureate 1944) > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:17 EST