Re: nucleated red blood cells

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> From:          "Kurtz, James" <KurtzJ@usa.redcross.org>
> To:            Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Date:          Fri, 5 Jan 2001 14:45:37 -0500

>
>
> Jim Kurtz
> Research Associate
> Jerome Holland Laboratory for Biomedical Sciences
> American Red Cross
> Product Development Dept.
> 15601 Crabbs Branch Way
> Rockville, MD 20855
> Ph#  301-738-0669
> Fx#  301-738-0553
> Email: jkurtz@USA.Redcross.org <mailto:jkurtz@USA.Redcross.org>
>
>
> I am interested in finding out any information regarding determining the
> nucleated red blood cell count using flow cytometry.
> Since there is interference from propidium iodide and since acridine orange
> can stain your tubing on your instrument, does
> anyone know another dye that can be used?
>
> Thankyou, Jim Kurtz
>
Try:  Tsuji, T., Sakata, T., Hamaguchi, Y., Wang, F. and Houen B. New
Rapid Flow Cytometric Method for the Enumeration of Nucleated Red
Blood Cells.  Cytometry 37: 291-301 (1999).

Greetings
>
Dr.H.Müller
Institut für Laboratoriumsmedizin
Klinik am Eichert Göppingen
Germany

holger.mueller@kae.de

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