If you say that percentages after thawing is greater than in fresh sample, the explanation is that mature cells are more fragile than hematopoietic ones and thus their number diminish during thawing procedure, thus increasing artificially CD34+ percentage. Ph. Bourin MD, PhD jglu@mail.mdanderson.org wrote : > In our BMT cell processing Lab, we routinely detect CD34 on PBPC after > collection(fresh PBPC) and before infusion(thawed PBPC). We often find CD34 > express in Thawed PBPC was higher than in fresh PBPC(same product). We use > ISHAGE method to calculate the CD34. We also use 7AAD to exclude the dead cells. > In the dot-plot(CD34PE vs SSC), we sometimes see denser or bigger population in > thawed PBPC(compare to fresh same PBPC). Anyone has same experience? what are > possible reasons to cause this? Is there any way to solve this problem and get > more accurate % CD34 and recovery? Thank you for your precious opinion. > > Jay
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