Depending on the mouse strain utilized, Cy5 can bind with an intensity anywhere from half a log to 3 and a half logs. I would repeat with a different fluorochrome before investigating further. Kb -- Science is built with facts as a house is with stones--but a collection of facts is no more a science than a heap of stones is a house. -Jules Henry Poincare (1854-1912) Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida Phone: (352) 392-4887 Fax: (352) 392-5393 kbahjat@ufl.edu on 12/21/00 07:29, Gregor Rothe at gregor.rothe@klinik.uni-regensburg.de wrote: > > Hello to all, > > in recent experiments in two different mice strains we were analyzing the > induction of > platelet-leukocyte aggregates as a function of platelet activation based on > staining > with CD41 and CD45. Interestingly, a strong rise in binding of CD45-PE/Cy5 was > associated > with platelet activation independent of the low number of platelet-leukocyte > aggregates > although CD45 at least in human is considered not to be expressed in > platelets. > > Looking through the literature, a cross-reactivity of CD45RA-antibodies with > an > intracellular antigen in murine platelets has been published. My question, > therefore, > is whether anyone observed a similar reactivity of CD45 clones with murine > platelets > or can provide addtional data proving real expression or an artefact. > > Regards, > > Gregor Rothe >
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