Hello Everyone, I actually have questions that are similar to the ones Regina has posed. However, I am trying to move a fluorescent microscopic assay to a Coulter XL. This is a clinical assay for granulocyte antibodies, so one of my most important tasks seems to be setting realistic cut-offs for positive reactions without losing weak reactions in the process. I am very interested in any recommendations you might have for Regina, and, if anyone has some experience in moving slide assays to flow cytometry- -no matter how long ago--I would be very interested. Thanks a million, Nan Cochrane Neutrophil Serology Laboratory North Central Blood Services St. Paul, MN -----Original Message----- From: Regina Harley [ mailto:rharley@medicine.umaryland.edu <mailto:rharley@medicine.umaryland.edu> ] Sent: Monday, December 18, 2000 2:01 PM To: cyto-inbox Subject: Multiple labs Flowers- We are setting up a flow lab in another country. We are planning on running samples both there and here in the US and are looking for ideas for QC and making sure the data generated by the two machines are comparable. Unfortunately, they are not the same instrument; in the US we have an Elite ESP and outside we'll have an XL (so at least they're both Coulter). Any suggestions for references or personal experience would be most welcome. The kinds of info I had in mind are: What kinds of beads do you run and how much of that daily QC info is it necessary to document? When running experiments, what kind of controls do you routinely run? What the best way of comparing data from two different instruments? Is there a panel (or set of panels) that we should use to do this? Is there an "official" way of doing this - i.e. something analogous to CAP certification? Thanks for any help/suggestions. cheers- Regina Regina H. Harley, M.S. Lab Supervisor - Flow Cytometry Facility Center for Vaccine Development University of Maryland - Baltimore 685 West Baltimore Street Baltimore, MD 21201 (410) 706-7376 FAX: (410) 706-6205 rharley@medicine.umaryland.edu <mailto:rharley@medicine.umaryland.edu>
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