Cell wall permeabilisation & fixation

From: Antonio de Padua Castillo Flores (acastilo@bgumail.bgu.ac.il)
Date: Sat Dec 16 2000 - 07:18:10 EST


Sorry, if same of you all ready read this e-mail. But since I did not receive any replay. I desire to send it once again. 

Hi, 
I am looking for protocols which are suitable to permeabilise and to
fixate microalgae cell wall (Haematococcus pluvialis). I am trying to
measure DNA per cell with a flow cytometer EPICS XL-MCL (Coulter Counter).


I will appreciated any kind of help or information.

Thanks
Antonio.

Antonio de Padua Castillo Flores
Aquaculture Eng., M.Sc. Student.

Microalgal Biotechnology Laboratory
The Jacob Blaustein Institute for Desert Research
Sde Boqer Campus. 84990 
Israel

Tel:  07 659 68 00
Fax: 07 659 68 02 



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