FlowJo is neat. but since I just started crackin' on the demo, I have a question I can't answer using just the help file. here it goes. I have some old burst test FACS files. They were acquired the wrong way - i.e. propidium iodide negative cells (dead) were stored (should be excluded during acquisition). The purpose of the burst test is to analyse the FL1 signal, which contains info about conversion of rhodamine to its fluorescent form. In order to pull the analysis off in cellquest I have to set my monocyte and granulocyte gates on Fl3 vs. eitherscatter dotplot. I would be much more comfortable using fsc. vs ssc. dotplot b/c in either fsc/ssc axis alone there is overlap between monos and grans (espeically after activation) So I thought, ok, I'll draw R1 on scatter vs. FL3 and include ALL the bright FL3 cells, make another dotplot (fsc vs. ssc) plot gated on G1 and then I can set "clean" gates on my mono's and gran's. Apparently I can't do that. (or can I? please let me know) So playing around with flowjo I noticed I can narrow populations as much as I would like to, even setting gates on histograms (how cool is that!) my problem is, now I don't know how to do cellquest's histogram M1 and M2 statistics in flowjo. is there a histogram stats tutorial on the web for flowjo? Or how about, after having gated on my FL3 histogram, can I create a new FACS file and analyse that in cellquest? as usual, I would appreciate any pointers. Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Yahoo! Shopping - Thousands of Stores. Millions of Products. http://shopping.yahoo.com/
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