Hi, As I requested an information for Krypton laser alignment beads, the following is a summary: There are several commercial beads, Yellow-green(from Polysciences), Sphero rainbow beads (cat # RFP-30-5A), and Molecular Probes's beads with catalog #P14826. Actually I tested rainbow beads and UV-beads form Molecular Probes, it worked greatly for 413 Krypton laser. Thank you for your great response. Liusheng > Liusheng He, MD, Ph.D > Flow cytometry Staff scientist > Director, Flow Cytometry Facility > Autoimmunity Branch > NIAMS, NIH > (301)594-3531 > Lihe@mail.nih.gov > ---------- > From: He, Liusheng (NIAMS) > Sent: Wednesday, November 22, 2000 2:27 PM > To: 'cytometry@flowcyt.cyto.purdue.edu' > Subject: commercial beads for 413 or 407 nm lines in Krypton Laser > > Hi, someone there: > Recently we tried to do Krypton laser alignment at 413 nm line for > FRET assay and found that Uv excited beads from Molecular Probe was not > excited by these lines. Is anyone there knowing any commercial standard > beads for line 413 or 407 nm? > Thank you for your helps. > Have a great thinkgiving. > > > Liusheng He, MD, Ph.D > Flow cytometry Staff scientist > Director, Flow Cytometry Facility > Autoimmunity Branch > NIAMS, NIH > (301)594-3531 > Lihe@mail.nih.gov >
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