mAb Titration

From: Yasmin Marikar (ycaldwel@com1.med.usf.edu)
Date: Wed Nov 29 2000 - 01:11:48 EST

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Flowers:
This is very basic question so please bear with me. What is the best/
most efficient way to titrate the mAbs I use for 4 color flowcytometry.
Can I combine them in groups consisting of four (FITC, PE, PerCP and
APC) at each defined concentration or do I have to run them all
individually?
Thankx
Yasmin

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