A few people asked for a summary of responses. Basically there were four possibilities: 1. Partec: Disposable filters from Partec. The internet site is www.partec.de; email is info@partec.de I see on our box that the options are 20, 30, 50, 100 and 150 micron sizes. We use the 50 um. The order number is 06-4-2317. We get them non-sterile and package and autoclave them if necessary. I hope this helps. 2. Falcon: I have always ordered the Falcon 2235 filter top tubes for this purpose. They are 12x75 and come with a 30 um filter fitted in the tube cap. They do not come sterile so I put a couple of tubes into each small sterilization bag and had our central sterile supply gas sterilize them for me. It worked great. 3. Miltenyi: I am using Pre-Separation Filters from Miltneyi Biotech Inc. with a mesh size of 30 micrometer. They fit into the tube but are a little wobbly. Order number is 414-07. The filters are sterile packed one in each package. I never had a problem with sorting after using this filter (at least concerning clogging of the 70 um nozzle). 4. BD: You can get these from BD. they are called Filcons. A bit expensive but I use them to sterile filter just before sorting. Check out their catalog. **************************************************************************** Elaine Kunze Flow Cytometry.....Image Analysis... Life Sciences Consortium 8B Althouse Laboratory (814-863-2762) Penn State University University Park, PA 16802
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:14 EST