I would not recommend to lyse whole blood but instead use gradient centrifugation to get rid of the RBC. We found that due to the large number of RBC in the blood there is often considerable clumping and thus cell loss associated with lysing blood. It is hard to get it right. We drip our mouse blood (tail bleeds) directly into about 1ml of PBS containing heparin. Each of the diluted blood samples is than overlayed onto "lympholyte M" which we get from Cedarlane Labs(Canada), but I am sure that Ficoll would work too. Nicole Baumgarth, DVM Ph.D. Center for Comparative Medicine University of California Davis, CA 95616 USA
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