Dear Colleaugues, we want to perform MHC II typing from peripheral blood in patients with suspected autoimmune disease. Being completely unexperienced in this field I have some very basic questions to the community. 1. Is it sufficient to stain the cells for HLA-DP, HLA-DQ, HLA-DR (I only found ABs to these subclasses) or do I need more antibodies? 2. Is it ueseful to use additionally an AB against the common HLA-D molecule? 3. Can I do all stainings in one well and use directly labelled ABs? 4. Where can I get HLA-DP directly dye labelled? 5. Should I gate on B-cells or monocytes? Does this matter at all? 6. What results should I expect: Is a patient either one of the HLA-D types or do I get a mix of all three types? 7. Anything important that should be taken into account? Any help is appreciated. Thanks Attila Tarnok
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:14 EST