Dear Flowers: There is a PI here that is trying to detect flourescence of a FITC labelled peptide that has been injected into live models. They are certain the peptide has entered the cells by the positive response to treatment, however it is not being detected by flow. It is, however, seen under a flourescent scope. They are seeing positive FITC when the assay is performed on cultured cells. They have said that this has worked for them in the past and are curious as to ideas on detection of intracellular flourescence using a FACSCalibur. If anyone can help it would be greatly appreciated. You can respond to me, I will relay the information. Thank you, Michele Nadaf
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