Snezna, You haven't had someone running acridine orange have you? Sounds like the fluorescence builds in the line and is flushed out by the sample. Frank. Frank Battye \ / < The Cytometry Laboratory Dora Kaminaris \__/ <<<<< The Walter & Eliza Hall Institute Viki Lapatis ------!!<<<<<<<< Victoria 3050, AUSTRALIA Jennie Chan /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852 Catherine Tarlinton o !! \ < in:: "facs_copy@wehi.edu.au" Geza Paukovics
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