>From: Len Brown <lbrown@mmri.mater.org.au> >To: "'Kerstin'" <beckk@sun11.ukl.uni-freiburg.de> >Subject: RE: specific DC-Marker >Date: Mon, 2 Oct 2000 11:42:38 +1000 >MIME-Version: 1.0 >Status: > >Dear Kerstin > >There are no "specific" markers for DC, this includes CD1a, which is in fact >a cortical thymocyte marker which is also expressed on some types of DC. DC >populations can be separated by FACS by their "lineage negative" >characterisation and high expression of HLA DR. Sub-populations of blood DC >express CD11c or CD123, while activated or mature blood DC express >co-stimulatory molecules such as CD83 and CD86 as well as our in-house >marker using the antibody CMRF44. > >As for CD34 or monocyte derived DC, these cells will express different >phenotypes depending on culture conditions and cytokines used. Langherhan >cells are well defined. > >Hope this helps. > >Len Brown >Mater Medical Research Institute >Brisbane, Australia > >-----Original Message----- >From: Kerstin [mailto:beckk@sun11.ukl.uni-freiburg.de] >Sent: Friday, September 29, 2000 2:42 AM >To: Cytometry Mailing List >Subject: specific DC-Marker > > > >Hello everybody, >does anyone know a specific marker (except CD1a) for Dendritic cells which >express mature and immature DCs? I want to seperate Monocytes, makrophages >and DC with FACS. Are there any differences in expression between >dendritic cells generated of CD34+progenitor cells, DC generated of >Monocytes or DC of the skin? >Thanks for your help. >Kerstin > >***************************************** >Kerstin Beck >Institut für Medizinische Mikrobiologie und Hygiene >Abteilung Virologie >Hermann-Herder-Strasse 11 >D-79104 Freiburg >Telefon : +49-(0)761-203-6646 >Fax : +49-(0)761-203-6603 >beckk@ukl.uni-freiburg.de >***************************************** > ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
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