I have a question concerning the usage of FACS for creation of monoclonal Schneider S2 cells and have been encouraged by Glenn Paradis at MIT to pose my question here. We have a poly clonal cell line expressing our protein of interest and GFP. Since the level of GFP should be roughly correlated to the amount of expressed protein, I would like to sort the high GFP expressers into monoclonal cultures. The problem is that the Schneider S2 cells can't grow when they are alone so sorting them into 96 well plates and waiting for them to proliferate is unfortunately out of the question. I have only found one reference describing the production of monoclonal lines, using mitomycin C treated feeder cells, and I therefore wonder if any of you should have experience with this or know of somebody who has. Please respond directly to my mailing address (JHR@me.dk) since I am not a part of the mailing list. I am grateful for any help that I can get. Regards Jakob H. Rasmussen (JHR@me.dk)
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