We have run calcium flux on isolated T cells and CD34 cells with SDF as stimulus. There is a resonable response to SDF by CD34 cells but not by T cells either CD4 or CD8. I am interested in any comments as to the non-response of T cells to SDF. We have repeated experiment 2 times and have loaded cells with Indo-1. There is no indication that loading of T cells with Indo-1 is a problem as they respond to ionomycin as a positive control. Thanks. David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Boston, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:11 EST