Scott, Algorithms used to fit these distributions often fail (or worse, give questionable results) because the histograms don't fit typical/expected patterns -- plus, % c.v.'s are often not very good. Therefore, I usually use histogram stats for analysis. Since most of the time you're interested in development of increasing ploidy (as opposed to modeling s-phase), I think this is sufficient. But . . . I believe ModFit has a peak fitting function, no? It is useful for fitting curves like those seen with CFSE or PKH staining. Check that out. MAK. Scott Tighe wrote: > Greetings fellow flowers > > Can anybody recommend the best way to model the cell cycle for budding > yeasts? I currently use ModFit 2.0 > for our cancer studies, but it does not lead itself to budding yeasts. > I am normally working with mammalian cells and do not have this trouble. > Is there a standard method to set-up these experiments when ultimately > one will need to model the yeast's cell cycle with curve-fitting > software? > > Sincerely > > Scott Tighe > Vermont Cancer Center > University of Vermont > 214A Medical Alumni Bd. > Burlington, VT 05405 -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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