I need to calibrate a partec PAS for the detection of E. coli, grown under starvation conditions in a chemostat (stained and unstain). I need to detect sizes from 1.0 um to 0.2 um. I used 0.2 um polystyrene beads from Polysciences, but they seems to become a resident particle in the tubing after their use. Any suggestions for particles that won't be left behind. Thank you Mark
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