Greatings again, In my samples from patients whose RBC's don't lyse, we use CD45 to gate out the lymphs. But, when I acquire, I get ~ 3000-5000 total events per second (my actual CD45 gate picks up only 100-200 events per second). I know I can set thresholds etc, to eliminate the non-CD45 stuff, but the fact that there are 4000 cells going through the lasers in addition to my 100 cells of interest each second makes me worry whether or not the Cytometer is keeping an accurate count. The model I use is the good ol' FACSCalibur. Input from folks at BD is especially appreciated. thanks a million (cells per sec) Maciej __________________________________________________ Do You Yahoo!? Yahoo! Messenger - Talk while you surf! It's FREE. http://im.yahoo.com/
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