Hello, I am pretty much a FACS beginner. I have a question, which nobody has been able to answer so far. Every time I do a FACS scan from peribronchial lymph nodes (naive BALB/c mice) and stain it for cd3, cd4 and cd8 cells I see a huge population of cd4pos/cd3neg cells, as well as cd8pos/cd3neg cells. Does anybody know what types of cells these are? I have to admit that isolating PBLN from naive mice is rather tricky, usually leaving me with extracting the entire hilus and meshing that. Could these cells derive from Thymus (which I am pretty sure to extract beforehand) or from oesophagus? Thank you very much, Ph. Stock
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:10 EST