Microbial Analysis at the Single Cell Level - Available On-Line

From: Howard Shapiro (hms@shapirolab.com)
Date: Mon Oct 23 2000 - 09:33:51 EST


The Journal of Microbiological Methods - Special Issue
Microbial Analysis at the Single-Cell Level
Volume 42, Number 1 (September, 2000): Available On-Line

This Special Issue, which I edited with Lilia Alberghina, Danilo Porro,
Friedrich Srienc, and Harald Steen, contains a representative selection of
work presented at a conference on "Analysis of Microbial Cells at the
Single Cell Level -Why, How, When?", organized by the Microbial Physiology
Section of the European Federation of Biotechnology, the Federation of
European Microbiological Societies (FEMS), the Società Italiana di
Microbiologia Generale e Biotecnologie Microbiche (SIMGBM), the Italian
Group of Cytometry (GIC), and the Universities of Milan, and held in Como,
Italy, March 25-27, 1999.  Abstracts of papers presented at the Conference
previously appeared in The European Journal of Histochemistry, Volume 43,
Supplement 1 (1999).


By agreement with Elsevier, the Publisher of The Journal of Microbiological
Methods, the full text and illustrations of all articles is now available
free of charge in Adobe Acrobat .pdf format at
http://www.elsevier.com/locate/jmicmeth

Contents

Editorial: Microbial Analysis at the Single-Cell Level
L. Alberghina, D. Porro, H. Shapiro, F. Srienc, H. Steen
pp.1-2 (1367.pdf)

Microbial Analysis at the Single-Cell Level: Tasks and Techniques
H. M. Shapiro
pp. 3-16 (1368.pdf)

FT-IR Microspectroscopy for Microbiological Studies
F.Orsini, D.Ami, A.M.Villa, G.Sala, M.G.Bellotti, S.M.Doglia
pp. 17-27 (1369.pdf)

Single-cell Analysis of Bacteria by Raman Microscopy: Spectral Information
on the Chemical Composition of Cells and on the Heterogeneity in a Culture
K. C. Schuster, E. Urlaub, J. R. Gapes
pp. 29-38 (1370.pdf)

A Glucoamylase:GFP Gene Fusion to Study Protein Secretion by Individual
Hyphae of Aspergillus niger
C.L. Gordon, D.B. Archer, D.J. Jeenes, J.H. Doonan, B. Wells, A.P.J.
Trinci, G.D. Robson
pp. 39-48 (1371.pdf)

Relating Growth Dynamics and Glucoamylase Excretion of Individual
Saccharomyces cerevisiae Cells
D. Porro, M. Venturini, L. Brambilla, L. Alberghina, M. Vanoni
pp. 49-55 (1372.pdf)

Real-Time Flow Cytometric Quantification of GFP Expression and
Gfp-Fluorescence Generation in Saccharomyces cerevisiae
P. De Wulf, L. Brambilla, M.Vanoni, D. Porro, L. Alberghina
pp. 57-64 (1373.pdf)

Flow Cytometry of Bacteria: Glimpses from the Past with a View to the Future
H. B. Steen
pp. 65-74 (1374.pdf)

Nucleic Acid-based Fluorescent Probes in Microbial Ecology: Application of
Flow Cytometry
J. Porter,  R. W. Pickup
pp. 75-79 (1375.pdf)

Gel Microdroplet Technique Leaving Microorganisms Alive for Sorting by Flow
Cytometry
T. Katsuragi, S. Tanaka, S. Nagahiro, Y. Tani
pp. 81-86 (1376.pdf)

Glucose Uptake Rates of Single E. coli Cells Grown in Glucose-Limited
Chemostat Culture
A. Natarajan, F. Srienc
pp. 87-96 (1377.pdf)

Analysis of Bacterial Function by Multi-Colour Fluorescence Flow Cytometry
and Single Cell Sorting
G. Nebe-von-Caron, P. J. Stephens, C. J. Hewitt, J. R. Powell, R. A. Badley
pp. 97-114 (1378.pdf)

This should be a useful reference for anyone interested in flow cytometry
of microorganisms, and my co-editors and I thank Elsevier for making it
available.

-Howard



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