Hi We are interested in identifying and sorting p-glycoprotein bearing cells. We thought that using a dye like rhodamine123 or Hoechst then sorting unlabelled cells would be OK, any thoughts? What are the dyes of choice for this sort of thing? I hear that the antibodies to g-gp aren't that good. It would make life easier if it were a 488 excited dye although UV or 633 would be OK. We want to use several phenotypic markers as well. Thanks for any help Simon Monard Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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