Dear Bassem, we published a paper a few years ago on epithelial cell dissociation from human intestinal biopsies. You can find the paper in : J. Immunol. Methods 158: 207-214 (1993). Authors: L. Madrigal et al. We used a combination of DDT (a reducing, mucolitic agent) and EDTA (a chelating agent that, by sequestering divalent ions, releases the epithelial cells). Several methods described for epithelial cell detachment in mice, rats and humans use a combination of these agents. In our hands, only epithelial layer was released, leaving intact the basement membrane and other cell layers. I don't know if it'll be useful for shellfish gills, but I imagine it would be worth your while trying. Best of luck, Laura Date sent: Fri, 13 Oct 2000 07:53:08 -0400 From: Bassem ALLAM <bassem.allam@univ-ag.fr> Subject: Cell dissociation media To: cyto-inbox > > Hi everyone, > We are looking for a cell dissociation media (or reagents) suitable for > the dissociation of epithelial tissue (shellfish gills which contains a > very thin connective layer). I'm sure that there is a way to apply (with > some modifications) media-protocols used for other models -mammals-. Any > inputs are welcome, Thank you. > > > \|/ > (o o) > ____________oOo__(_)__oOo_____________ > Bassem ALLAM > Laboratoire de Biologie Marine > Université Antilles-Guyane - UFR Sciences > B.P. 592 > 97159 Pointe-ŕ-Pitre Cedex > FRANCE > > Phone: (0) 590 93 87 25 > Fax: (0) 590 93 87 24 > email: bassem.allam@univ-ag.fr > ______________________________________ > > ************************************************************************ Laura Madrigal-Estebas, PhD Research Laboratories Coordinator Education and Research Centre St. Vincent's University Hospital Elm Park Dublin 4 IRELAND Tel: 353-1-2094940 FAX: 353-1-2838123 e-mail: Laura.Madrigal-Estebas@ucd.ie
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:09 EST