I wanted to do a "test" run on new lot of antibodies we're going to use for an intracellular il-10 measurment in monos (see previous posts). When it was all done, I found that CD33 was nowhere to be seen in tubes activated with PMA (4 hrs). CD14 was there just fine. I was expecting it to be the other way around.. I didn't use LPS b/c I know that will block/shed my CD14's. With other activators I used today I had normal results, so only the PMA raised an eyebrow. This is the first time I used PMA to check cytokine production. my PMA came from leftover bursttest kits by orpegen, I used a modest .02mM final conc. I wonder if anyone had similar findings or any words of wisdom? Maciej __________________________________________________ Do You Yahoo!? Yahoo! Messenger - Talk while you surf! It's FREE. http://im.yahoo.com/
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