Dear Colleagues: I really hate to ask this but we are in an urgent need of a mammalian expression vector of CD20. I need this because I would like to identify and gate cells that have been transfected with a DNA of interest. In another word, I would like to use CD20 as a transfection marker. I tried first with EGFP, yet this appears to be associated with some cell toxicity and not suited for a kind of experiments I am planning to do, ie apoptosis assay. CD20 can be easily detected after paraformaldehyde fixation. I would be very much appreciative if you could share that with us. Ken Fujise, MD Research Center for Cardiovascular Diseases Institute of Molecular Medicine, Houston TX
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