Hi Flowers Here's one for the clinical flow cytometrists - how do you separate the cells from a slimy snotty goopy bronchial lavage ( have I totally put off the non-clinical cytometrists yet?). I have tried diluting it and flushing it through a 100um filter, but as soon as I centrifuge it the gunk just condenses again. The microbiologists use something called mucolyse (dithiothreitol) which reduces mucoprotein disulphide bonds, but I'm a bit scared to use this in case it interferes with the cell surface in any way. Any suggestions much appreciated. beth.
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