I am searching for a good protocol to culture CD68+ macrophages from relatively pure monocyte populations . The macrophages should be relatively unactivated. Several protocols are described in the literature varying from culturing with autologous or AB+ serum to use of M-CSF. Can anyone suggest me a method which yields pure (>90%) macrophages to be prepared from maximum 30 ml of heparinized human blood? Thanks in advance, Mathilde Kouwenhoven ------------------------------------------------------------------------------ Volkan Özenci Division of Neurology Karolinska Institutet Huddinge University Hospital Stockholm, Sweden Tel: +46 8 58582278 Fax: +46 8 58587080
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