We are looking CD 11b expression on neutrophils during cardiopulmonary bypass surgery. At various times during the surgery, the neutrophils are exhibiting bimodal expression which is evident in the histogram of CD 11b positive events. We suggest this represents two distinct populations of neutrophils that are expressing CD 11b. My two questions are: 1. Why are the neutrophils showing bimodal expression. 2. Which population of neutrophils should we gate on, if not both populations. Thanks, Chad Brouse
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