Greetings floweurs: Is streptavidin-CyChrome (aka PE-Cy5) too big to get into the nuclei of ethanol-fixed tumor cells? I stained a couple colon tumor lines (HT-29 and HCT-116) with mpm2-biotin (a marker of mitosis) + SA-CyChrome and didn't see anything. It worked fine with plain mpm2 antibodies + goat anti-mouse-FITC, but I need green for something else. BrdU antibodies conjugated to PE get into the nucleus of ethanol-fixed cells OK, but the cells are also usually treated with acid. Do I need bigger holes in the membrane(s) or should I look elsewhere for an explanation? Ken Hook Pfizer Global R&D Ann Arbor
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