Storage of PMNC from coord blood

From: Corrado Cilio (corrado.cilio@cmb.umu.se)
Date: Fri Sep 08 2000 - 05:06:02 EST


Hi everybody,

I am in process to receive a large number of cord blood samples and I would
like to freeze PMNC for later analysis by flow cytometry after in vitro
activation.

I would appreciate any suggestion regarding best freezing media,
conditions, thawing protocol etc.

Thank you very much for any help!

Corrado



		      ''''''''
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Corrado M. Cilio, M.D., Ph.D.
Wallenberg laboratory
Malmo University Hospital
Lund University
S-205 02 Malmo, Sweden
Tel: +46-40-332395; mobil: +46-70-4330338; Fax: +46-40-337042
e-mail: corrado.cilio@cmb.umu.se
 --------------------------------------------------------------

Judge a man by his questions rather than his answers.
 - Voltaire -



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