Hello again Thankyou for all your suggestions, I have various protocols to try now... Thanks again, Louise Dr. Louise Affleck Senior Scientist Protein Function Group AdProTech Ltd Unit 3, 2 Orchard Road Royston Herts SG8 5HD Tel: +44 (01763) 226090 Fax: +44 (01763) 226021 http://www.adprotech.co.uk ----- Original Message ----- From: "Louise Affleck" <l.affleck@adpro.co.uk> To: cyto-inbox Sent: Thursday, August 31, 2000 11:40 AM Subject: Isolation of B cells from whole blood > > Hello fellow flowers, > > I don't have access to a sorter or a MACS but I need to isolate B cells > (ideally CD21+) from, rabbit, marmoset, and rhesus monkey whole blood. I've > thought about coupling human C3d (the ligand to CD21) to sepharose then > passing either buffy coat or lysed blood prep, washing the unbound CD21- > cells and eluting the CD21+ cells... Any other suggestions would be > appreciated. If MACS or sorting is the only other possible way then I could > probably get access to these pieces of kit....any suggested protocols would > be greatly appreciated.. > > Thanks, > > Louise > > Dr. Louise Affleck > Senior Scientist > Protein Function Group > AdProTech Ltd > Unit 3, 2 Orchard Road > Royston > Herts > SG8 5HD > Tel: +44 (01763) 226090 > Fax: +44 (01763) 226021 > http://www.adprotech.co.uk > >
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