> There is an effect that has been observed regarding the CV of 2.5 micron > calibration beads using on a flow cytometer. When the peak channel is located at > lower channel numbers the CV is increased. i.e. the CV of a population is higher > at mean channel 100 ( 2.41) than at mean channel 800 ( 1.25). What is the > statistical explanation for this phenomenon. Hello Robert, Statistics for a histogram are calculated as follows: CV = (SD/Mean) x 100 where Mean = [SUM(channel number x count in the channel)]/total number of cells in the region. So higher channel numbers give higher Mean and - finally - lower CV. I think it works for any kind of particles. I hope this helps you. Best regards, Michal Michal Bochenek, PhD. mbochen@izoo.krakow.pl National Research Institute for Animal Production Dept. of Animal Reproduction 32-083 Balice/Krakow, Poland ph. (48 12) 2856777 ext. 145 fx. (48 12) 2856733
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