Hello, If you want your platelets out of the way (as clumps) then Heparin is your choice, the specimen has bluish back ground if you want look at it under the microscope after a Romanowski stain. EDTA is working fine with me with both blood and bone marrow. The jelly like marrow may be due to (not enough anti coagulant!). You can always treat it a solid sample and pass it through a mesh and suspend in PBS or sheath fluid. There is preparation that is used to remove wartan jelly from cord blood but I do not remember who makes it. It may be useful but I have not tried it! Regards Fareed Al-Gurg Dubai Hospital Dubai-UAE. Virginia M Litwin wrote: > All: > I am forwarding this question that I received regarding bone > marrow samples. I have no experience with bone marrow. Can > anyone respond to Judith's question? > Thanks, > Virginia > > ---------------------------------------------------------------- > > Subject: Re: (no subject) > Date: Wed, 30 Aug 2000 01:25:14 +0300 > From: "j. chezar" <javicn@netvision.net.il> > To: cyto-inboxHi I read your comments on ACD in the Purdue message > board. I wonder if you can explain this. I work on blood and bone > marrow specimens, usually with EDTA. Blood is no problem but bone > marrow can really be awful. Occasionally, like today, a bone marrow > sample that i got from antoher hospital looks like it has jelly- fied. > it is not clotted. just STICKY as can be and pretty impossible to work > with. Since i got the sample still in the syringe (with a cap on it) i > strongly suspect the anticoagulant is heparin. because bone marrow is > not an easy procedure i tried to salvage some cells. I usually q-prep > or ammoniaum chloride, but this time only ficol saved a few but most > of the cells were gone. questions:what anticoagulant do you prefer for > bone marrow samples?have you come accross this phenomenon and can you > explain it.is there anything i can do to salvage the sample. thanks > for your time judith chezarIsrael
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:03 EST