Dear steve
Are you manually staining? If so longer incubation time can increase signal,
so can a lower dilution of the primary antibody, give me some more of your
situation and I will be glad to help, have been doing IHC about 10 yrs. If
you want to call and discuss at length let me know.Dana Dittus
Histlogy Supervisor
Abington memorial Hospital
215-481-2609
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