Back to CD4/CD8

From: Liza McGuire (l_mcguire@hendrix.jci.tju.edu)
Date: Fri Aug 25 2000 - 17:05:15 EST

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Hi Flowers,

This time the question refers to CD4+CD8+ cells?
Has anyone observed a significant double positive population on Neupogen stimulated PBSCs? The cells are CD3+/CD4+/CD8+ of which 10% of the total CD3+ cells are CD4+CD8+. We gave a quick look at the scatter of the cells and they are the same size as all T cells (smack in the middle of the lymph gate). We looked at the cells morphologically by staining them and could not see any immature and/or abnormal cells (normal diff). Any ideas? I appreciate any thoughts on the matter.
Thank you,
Liza McGuire

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