on 22.08.2000 2:00 Uhr, Sue Moss at s.moss@medicine.unimelb.edu.au wrote: Dear Suzanne Moss, If you quickly want to have the infected erythrocytes, you may also make use of an alteration in the magnetism of the infected red blood cells. The infected stages have a high content of haemozion, which allows them to be retained in the magnetic field in a MACS magnetic column. The high gradient magnetic field used by MACS technology is strong enough. Mature forms of Plasmodium falciparum infected erythrocytes (late trophozoites and schizonts) can be isolated by separation over a C Column using a 0.9x40mm (20 G) flow resistor. There is a nice reference on this: Detection of Antibodies to Variant Antigens on Plasmodium falciparum -Infected Erythrocytes by Flow Cytometry. Staalsoe T et al., Cytometry 35: 329-336, 1999. If you have further question please do not hesitate to contact us. I hope this helps Hans-Peter -- Dr. Hans-Peter Steffens Technical Support Miltenyi Biotec GmbH Friedrich-Ebert-Str. 68 51429 Bergisch Gladbach Tel: +49-2204-8306-264 Fax: +49-2204-85197 http://www.MiltenyiBiotec.com/ > > Hello Flowers, > we have a new Professor who has joined our Dept and he would like us to run > malaria infected rbc's stained with EtBr and FITC (secondary antibody) on our > cytometers (initially on the analyser and possibly sorting later on). We do > not have any experience with this type of work so I was wondering if anyone > had > any suggestions regarding safety precautions, waste disposal and also any > pointers regarding fixation protocols they would be greatly appreciated. > Thanks in advance, Sue. > Suzanne Moss (PhD.) > Senior Research Officer > Inflammation Research Centre > Department of Medicine > Royal Melbourne Hospital > The University of Melbourne, Parkville, Vic, 3052 > Email: s.moss@medicine.unimelb.edu.au > Phone: 61 3 8344 6252 > Fax: 61 3 9347 1863
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