Hi, This isn't a flow question but perhaps there are some labs out there doing both flow and fluorescence image analysis. We will soon receive an upright Leica TCS SP2 microscope and those investigators who have been using our ACAS (which uses an inverted microscope) for analysis of viable cells in a closed system with the 100x oil objective are going to have to find a way to do this with the upright. So my question: Is anyone doing fluorescence analysis on viable cells using an upright microscope and the 100x oil objective, i.e., the cells will have to be upside down on the upper cover glass and the chamber filled with culture fluid. If so, did you have to make your culture holder or are there commercially available systems that will accomplish this? The Bioptechs catalog showsthe FCS3 which would permit this but I'm not sure if it's in production yet. Thanks for any help. Ray Raymond B. Hester, Ph.D. Research Cytometry Laboratory CSAB 357, Biotechnical Services University of South Alabama Mobile, AL 36688 Voice: (334) 460-6029 FAX: (334) 460-6073 E-mail: rhester@jaguar1.usouthal.edu
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