Diana, Carleton Stewart spoke in detail about commercial antibody quality and preparation at the Flow course at Bowdoin College this June, but I can't find any specifics in my notes. I remember Carl stating how important it was to centrifuge your antibodies prior to use. Perhaps someone else who was in attendance could come up with these numbers, etc.?? Sue Dawes Internet Mail Message Received from host: flowcyt.cyto.purdue.edu [128.210.159.11] Envelope Sender: owner-cyto-sendout@flowcyt.cyto.purdue.edu From: "Diana Martin" <zigra@hotmail.com> on 08/14/2000 11:38 AM To: cyto-inbox cc: (bcc: Susan Dawes-SM/PGI) Subject: Does anyone ultracentrifuge their labeled primary antibodies or MHC tetramers before use in cell surface staining? If so, can someone pass along what conditions you use (ie how many g's and for how long)? We have a desktop airfuge. I can't find a chart anywhere telling what size proteins pellet at what speed. If anyone has this info, I would greatly appreciate it. Thanks Diana Martin Post-doctoral Fellow Center for Tropical and Emerging Global Diseases University of Georgia ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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