If EDTA is not washed quickly washed away after detactment the cells change size. Frank Radella II MS Tel (206) 341-5377 Pulmonary Research Labs FAX (206) 341-5376 R&T Building Rm 618 Box 359762 Page (206) 540-4553 On Wed, 9 Aug 2000, Robert Auerbach wrote: > > > Response to Thanos Kakoulidis > > EDTA works on most adherent endothelial cells, but takes time. TIming is > critical because the cells are not altogether happy. We have on occasion > (e.g. for porcine embryonic endothelial cells) used EDTA + collagenase. > > Bob Auerbach > > > > > > At 07:09 PM 8/7/00 -0400, you wrote: > >Hi Flowers, > > > >does anybody know of another method except trypsinization, to detach > >endothelial cells from the flask? (that is necessary in order to create a > >single cell suspension and run the cells in the flow cytometer) > > > >thanks, > > > >thanos > > > >Thanos Kakoulidis, MD > >Ph.D. Graduate student > >Department of Microbiology and Immunology > >Institute for Cellular Therapeutics > >University of Louisville > > Robert Auerbach > Laboratory of Developmental Biology > University of Wisconsin > 1117 West Johnson Street > Madison, WI 53706 > Phone: 608 262 1789 > FAX 608 262 1171 > e-mail rauerbac@facstaff.wisc.edu > (formerly auerbach@macc.wisc.edu) > >
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