I am investigating antigen uptake in murine cells and I am wanting to use Time vs fluorescence (on a Coulter Elite flow) as the parameters. The dyes that we are currently using are Lucifer Yellow and FITC-Dextran, and although these are good indicators of uptake, they seem to fluoresce in solution so that I cannot discriminate between active uptake and what is in solution. What I really need is a membrane impermeable dye that fluoresces once internalised. Does anyone have any ideas of what other dyes I can use or references (i have been looking through the Molecular Probes handbook) or what possible mistakes I could be making. Thanks Bernadette Bellette PhD student. -- Mark A Cozens Division of Pathology University of Tasmania ph 03 62264828 fax 03 62264833
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