Hi folks, We appreciate the interest expressed in our new enzyme products but want to clarify their source. The cell detachment and cell/tissue dissociation reagents that Sam refers to are actually available through Innovative Cell Technologies, Inc. (www.innovativecelltech.com), rather than from the parent company, Phoenix Flow Systems, Inc. Best Regards, Jeff Harvey Phoenix Flow Systems/Innovative Cell Technologies ----- Original Message ----- From: Witherspoon, Sam <sw11527@glaxowellcome.com> To: cyto-inbox Sent: Tuesday, August 08, 2000 2:22 PM Subject: RE: detaching EC > > Hi, > Folks I work with have successfully detached murine and rat > endothelial cells with collagenase (since these cells were grown on a > collagen matrix). These cells maintained their "stainability" for CD31. > > Am interested in but have not yet tried the reagents available at > Phoenix Flow Systems: > http://www.phnxf > Cheers, > Sam > Sam Witherspoon > sw11527@glaxowellcome.com > > Dept. of Receptor Biochemistry Tel. 919-483-3078 > Glaxo Wellcome R&D Page 919-857-7768 > 5 Moore Dr. Fax 919-483-0585 > RTP, NC 27709 > > > -----Original Message----- > > From: Thanos P Kakoulidis [SMTP:kakoulidis@louisville.edu] > > Sent: Monday, August 07, 2000 7:10 PM > > To: Cytometry Mailing List > > Subject: detaching EC > > > > Hi Flowers, > > > > does anybody know of another method except trypsinization, to detach > > endothelial cells from the flask? (that is necessary in order to create a > > single cell suspension and run the cells in the flow cytometer) > > > > thanks, > > > > thanos > > > > Thanos Kakoulidis, MD > > Ph.D. Graduate student > > Department of Microbiology and Immunology > > Institute for Cellular Therapeutics > > University of Louisville > >
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