Bill We have had some success using saponin permeabilisation and To-pro3 (Molecular Probes) albeit not with cardiac smooth muscle cells. (Of course you need 633/635nm 'ish excitation for To-pro3) Ian Ian Titley PhD Leukaemia Research Fund Centre at the Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB UK Tel Direct: +44 (0)20 7970 6048 or Tel: +44 (0)20 7352 8133 ext5134 Fax: +44 (0)20 7352 3299 E-mail: iant@icr.ac.uk On Tue, 8 Aug 2000 13:57:53 -0500 William Ross <wgr8w@virginia.edu> wrote: > > what's a good dye to use for simultaneous DNA and GFP detection when your > UV laser has taken an extended vacation? cardiac smooth muscle cells. > > Much thanks. > > Bill Ross > > William (Bill) Ross > Director - FACS Core Facility > MR-4, Box G005 > University of Virginia School of Medicine > Charlottesville, VA 22908 > office (804) 982-1586 > fax (804) 924-1221 > e-mail:wgr8w@virginia.edu > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:59 EST