>Hi Bill, >I have used the method published by Chu in Cytometry 36, 333-339 (1999), >bascically ice-cold 1% PFA incubated with cells on ice for 1h then the 70% >ethanol step etc and finally PI. I have found that for some cell types you >do get leakage, COS cells were fine. > > >Gary Warnes, >Head of Flow Cytometry at Fluorescent Imaging >MRC, Hammersmith Hospital, >London, UK
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