Fw: trypan blue to reduce background

From: Geneva M. Omann (gmomann@umich.edu)
Date: Fri Jul 28 2000 - 09:40:43 EST 

Hello Flowers,
    Along the lines of Shannon Ritchie's question below, I have also heard
of using trypan blue or crystal violet to quench the fluorescence of
externally bound ligands.  I believe the mechanism is by energy transfer.
Has anyone else heard of this and if so do you have references for the
mechanism and protocols?

----- Original Message -----
From: Shannon C. Ritchie <sritchi@emory.edu>
To: cyto-inbox
Sent: Wednesday, July 26, 2000 1:42 PM
Subject: trypan blue to reduce background


>
> I have heard of using trypan blue to reduce background.  Does anyone know
> the concentration and protocol?
>

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