Dear all, Joan Kalnitsky enquired about viable staining of potato protoplasts with either DAPI or Hoechst 33342. I seem to remember a paper on this subject: Hoechst 33258 as a vital stain for plant cell protoplasts. Meadows MG and Potrykus I Plant cell Rep. 1:77-79 (1981) However, when I tried this on several plant species, I could not obtain both viable and QUANTITATIVE staining. Best wishes Nigel Blackhall Experimental Officer, Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD. Tel 0115 9515151 x 18501 Fax 0115 9513298
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