Hello all, I have question in regard of 2-dodecyl resorufin form ImaGene Red C12RG lacZ kit (Molecular probes). I can see stained E. coli under the microscope and detect fluorescence on fluoremeter... no problem!! and it is specific to cells but have no signal on FACS or used 513, 568 and 590 excitation lines and bunch of different filters. Does any one has experience with it? Thanks a lot in advance, Artem
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